Search for: All records

Spontaneous epimutations—stochastic changes in cytosine methylation—can persist across generations in plants and are thought to contribute to phenotypic variation. Although epimutations are increasingly studied for their potential long-term effects, it remains unclear why their accumulation varies across genotypes. Here, we tracked DNA methylation across ten generations in ~400 mutation accumulation lineages derived from ~70ArabidopsisLer × Cvi recombinant inbred lines. Treating epimutation rates as quantitative molecular traits, we mapped a major QTL to a Cvi-derived deletion nearVIM2andVIM4, two genes involved in CG methylation (mCG) maintenance. We show that this deletion rapidly reduces genome-wide methylation to a lower steady-state and compromises mCG maintenance fidelity across generations, resulting in a ~1.5-fold increase in epimutation rates. Genotypes with elevated rates exhibited accelerated epigenetic drift and phenotypic divergence. Our findings support a punctuated-equilibrium model of mCG evolution, in which sudden disruptions to methylation homeostasis can destabilize epigenetic inheritance over longer time-scales. 

The rapidly intensifying effects of climate change on urban settlements demand that cities move to the forefront of resilience planning. Climate extremes, from heatwaves to flooding, are increasingly testing the adaptability limits of urban systems and the vulnerability of their populations. Recognizing the unique position of cities, the IPCC’s seventh assessment cycle has prioritized urban areas in its upcoming Special Report on Climate Change and Cities. The IPCC report underscores the potential of cities to act as agents of climate adaptation and provides a framework for cities to build climate-resilient systems. Cities are positioned to pioneer practical, integrative solutions that blend climate sciences with urban planning, establishing frameworks that align economic growth, health equity, environmental sustainability, social justice, and effective governance. This opinion piece explores how cities, by positioning themselves as hubs for innovation, policy reform, and community collaboration, can transform climate vulnerabilities into opportunities for community resilience and sustainability, especially by becoming more-than-human cities, setting examples on the global stage. 

The IPCC’s Special Report on Climate Change and Cities shows how cities must adapt to climate risks. Urban planners need to create solutions that fit each city’s needs, enhancing urban adaptability and resilience in the context of increasing climate-related risks. Sustainable urban planning, increased citizen awareness, and resilient infrastructure design are crucial in mitigating the growing impacts of climate change on human settlements. Addressing these challenges requires the integration of perspectives from diverse disciplines, including the natural sciences, social sciences, and engineering fields. This article draws on insights from a collaborative effort among experts in these areas, promoting a more coordinated and interdisciplinary approach. By bridging this expertise, we aim to advance resilience practices and awareness, fostering effective urban climate solutions in Texas and beyond. 

Heterochromatin is critical for maintaining genome stability, especially in flowering plants, where it relies on a feedback loop involving the H3K9 methyltransferase, KRYPTONITE (KYP), and the DNA methyltransferase CHROMOMETHYLASE3 (CMT3). The H3K9 demethylase INCREASED IN BONSAI METHYLATION 1 (IBM1) counteracts the detrimental consequences of KYP-CMT3 activity in transcribed genes.IBM1expression inArabidopsisis uniquely regulated by methylation of the 7th intron, allowing it to monitor global H3K9me2 levels. We show the methylated intron is prevalent across flowering plants and its underlying sequence exhibits dynamic evolution. We also find extensive genetic and expression variations inKYP,CMT3, andIBM1across flowering plants. We identifyArabidopsisaccessions resembling weakibm1mutants and Brassicaceae species with reducedIBM1expression or deletions. Evolution towards reduced IBM1 activity in some flowering plants could explain the frequent natural occurrence of diminished or lost CMT3 activity and loss of gene body DNA methylation, ascmt3mutants inA.thalianamitigate the deleterious effects of IBM1.